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mda assay kit  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)

 
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    Nanjing Jiancheng Bioengineering Research Institute Co Ltd mda assay kit
    Mda Assay Kit, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+kit/assay+kit+mda/pm42321347-195-14-24
    Average 86 stars, based on 1 article reviews
    mda assay kit - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: HIF1A transcriptionally activates CDKN1A to drive ferroptosis in skeletal muscle ischaemia-reperfusion injury.
    Article Snippet: .. The relevant kit manufacturers and their product codes are as follows: MDA Kit (A003-1-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), GSH Kit (A006-2-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), ROS assay kit (JS1323-Mu, Jinma Biological Engineering Co., Ltd., Shanghai, China), and Iron assay kit (ml095089, mlbio, Shanghai, China). ..

    Article Title: Radiotherapy followed by metformin neoadjuvant therapy drives superior pancreatic cancer ferroptosis and augments anti-tumor immunity
    Article Snippet: .. GSH and MDA assay GSH and MDA levels of cancer cells or tumor tissues were measured ith a GSH kit (A006-2-1, Nanjing Jiancheng Bioengineering Institute, hina) or MDA kit (A003-1-2, Nanjing Jiancheng Bioengineering Insti- ute, China), respectively. ..

    Article Title: Morin alleviates sepsis-associated encephalopathy through inhibiting ferroptosis via SIRT1.
    Article Snippet: .. The MDA expression of hippocampus was detected with MDA kit (Nanjing Jiancheng, China). ..

    Article Title: Astragaloside IV Alleviates Osteoarthritis by Inhibiting Chondrocyte Ferroptosis via the p53/SLC7A11/GPX4 Axis
    Article Snippet: .. Then, use the MDA kit (Nanjing Jiancheng Bioengineering Institute, Cat# A003-1) and GSH kit (Nanjing Jiancheng Bioengineering Institute, Cat# A006-2-1) to detect the contents of MDA and GSH. ..

    Article Title: Bicyclol improves cognition deficits and inhibits oxidative stress-induced neuronal cell apoptosis in alzheimer's disease via Nrf2/HO-1 pathway.
    Article Snippet: Extended author information available on the last page of the article Abstract Alzheimer’s disease (AD) is identified as the prevalent neurodegenerative condition globally, ultimately resulting in dementia.. Currently, the mechanisms that contribute to AD are not well comprehended, and there are few therapeutic alternatives available.. Bicyclol, a substance extracted from the Chinese herb Schisandra Chinensis, has shown remarkable antioxidant, anti-inflammatory, anti-apoptotic, and neuroprotective characteristics.

    Article Title: Ginsenoside Re attenuates homocysteine-induced endothelial cell ferroptosis through upregulation of GPX4/xCT signaling
    Article Snippet: .. To measure MDA content using an MDA kit (cat. no. A003-4-1; Nanjing Jiancheng Bioengineering Institute), 100 μl anhydrous ethanol, standard solution and test samples were mixed separately with 1,000 μl working solution to prepare control tubes, standard tubes and sample tubes, respectively. ..

    Article Title: Clostridium butyricum and its metabolite spermidine delay cognitive decline during natural brain aging by reducing inflammation and oxidative stress.
    Article Snippet: .. A portion of the supernatant was analyzed according to the instructions of the MDA Kit (A003–1; Nanjing Jiancheng, China). ..

    Article Title: HIF1A transcriptionally activates CDKN1A to drive ferroptosis in skeletal muscle ischaemia-reperfusion injury
    Article Snippet: .. The relevant kit manufacturers and their product codes are as follows: MDA Kit (A003-1-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), GSH Kit (A006-2-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), ROS assay kit (JS1323-Mu, Jinma Biological Engineering Co., Ltd., Shanghai, China), and Iron assay kit (ml095089, mlbio, Shanghai, China). ..

    ROS Assay:

    Article Title: HIF1A transcriptionally activates CDKN1A to drive ferroptosis in skeletal muscle ischaemia-reperfusion injury.
    Article Snippet: .. The relevant kit manufacturers and their product codes are as follows: MDA Kit (A003-1-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), GSH Kit (A006-2-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), ROS assay kit (JS1323-Mu, Jinma Biological Engineering Co., Ltd., Shanghai, China), and Iron assay kit (ml095089, mlbio, Shanghai, China). ..

    Article Title: HIF1A transcriptionally activates CDKN1A to drive ferroptosis in skeletal muscle ischaemia-reperfusion injury
    Article Snippet: .. The relevant kit manufacturers and their product codes are as follows: MDA Kit (A003-1-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), GSH Kit (A006-2-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), ROS assay kit (JS1323-Mu, Jinma Biological Engineering Co., Ltd., Shanghai, China), and Iron assay kit (ml095089, mlbio, Shanghai, China). ..

    Iron Assay:

    Article Title: HIF1A transcriptionally activates CDKN1A to drive ferroptosis in skeletal muscle ischaemia-reperfusion injury.
    Article Snippet: .. The relevant kit manufacturers and their product codes are as follows: MDA Kit (A003-1-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), GSH Kit (A006-2-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), ROS assay kit (JS1323-Mu, Jinma Biological Engineering Co., Ltd., Shanghai, China), and Iron assay kit (ml095089, mlbio, Shanghai, China). ..

    Article Title: HIF1A transcriptionally activates CDKN1A to drive ferroptosis in skeletal muscle ischaemia-reperfusion injury
    Article Snippet: .. The relevant kit manufacturers and their product codes are as follows: MDA Kit (A003-1-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), GSH Kit (A006-2-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), ROS assay kit (JS1323-Mu, Jinma Biological Engineering Co., Ltd., Shanghai, China), and Iron assay kit (ml095089, mlbio, Shanghai, China). ..

    Expressing:

    Article Title: Morin alleviates sepsis-associated encephalopathy through inhibiting ferroptosis via SIRT1.
    Article Snippet: .. The MDA expression of hippocampus was detected with MDA kit (Nanjing Jiancheng, China). ..

    Control:

    Article Title: Ginsenoside Re attenuates homocysteine-induced endothelial cell ferroptosis through upregulation of GPX4/xCT signaling
    Article Snippet: .. To measure MDA content using an MDA kit (cat. no. A003-4-1; Nanjing Jiancheng Bioengineering Institute), 100 μl anhydrous ethanol, standard solution and test samples were mixed separately with 1,000 μl working solution to prepare control tubes, standard tubes and sample tubes, respectively. ..



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    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid <t>peroxidation</t> (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde <t>(MDA)</t> levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid <t>peroxidation</t> (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde <t>(MDA)</t> levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid <t>peroxidation</t> (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde <t>(MDA)</t> levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    Image Search Results


    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: Microenvironment-educated MSC-EVs loaded injectable smart hydrogel for targeting senescent nucleus pulposus cells and inhibiting ferroptosis against intervertebral disc degeneration

    doi: 10.1016/j.bioactmat.2026.02.030

    Figure Lengend Snippet: D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: The level of lipid peroxidation was measured by Lipid Peroxidation MDA Assay Kit (Beyotime, China) according to the manufacturer's protocol.

    Techniques: Western Blot, Staining, Control, Flow Cytometry